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Effect of pH on the spectra of isomerase·flavin complexes at 37°C. Part a. Spectra of enzyme·FMNH− (red) and enzyme·FMNH2·IPP (dark green) at pH 7.0 and enzyme·FMNH2·IPP (light green) at pH 8.5. Part b. Spectra of enzyme·FMNH−·NIPP at pH 7.0 (pink) and 8.5 (cyan).
