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EPR spectra of enzyme·FMNH.·DMAPP at 77 °K produced by reduction ( pH 7.7, 25°C) of enzyme·FMN·DMAPP in H2O (dark purple) and D2O (light purple). Spectrometer settings were as follows: microwave frequency, 9.48 GHz; microwave power, 20 µW; modulation frequency, 100 kHz; modulation amplitude, 1 G. The linewidths are 19 G in H2O and 15 G in D2O. The g factor was 2.0046 based on a TEMPO standard.
