Table 1.

Steady state kinetic parameters for type II IPP isomerases

Enzyme	IPP			FMN	NADPH	MgCl2
	kcat × 102 (s−1)	Km (µM)	kcat/Km × 103 (µM−1s−1)	KD (µM)	KD (µM)	KD (µM)
T. thermophilusa	17.9 ± 0.2	5.6 ± 0.3	32	4.69 ± 0.55	110 ± 10	130 ± 10
B. subtilisb	25	670	nr	nr	nr	nr
Synechocystisc	23	52	4.4	nr	nr	nr

(nr) Not reported

^aAssay conditions for IPP parameters: 200 mM HEPES pH 7.0, 6 – 10 nM T. thermophilus type II IPP isomerase, 1 – 40 µM [¹⁴-C] IPP (10–59 µCi/µmol) 40 µM FMN, 2 mM NADPH, 2 mM MgCl₂, 0.14 mg/ml BSA, 37 °C.

^bRef. 29; k_cat calculated from the reported V_max.

^cRef. 28.