Table 2.

Increased sensitivity of triple-stain method over hematoxylin and eosin (H and E) in identifying occurrences of vessel invasion by tumor cells

			Paired differenceb
	Triple stain	H and E	Mean ± SE	95% CI for mean	p
Patients presenting with angio/lymphatic vessel invasiona	33	9			0.88
Number of invasive eventsc	102	13	1.85 ± 0.38	1.09 to 2.62	<0.001
Intratumoral events	48	4	0.92 ± 0.26	0.40 to 1.44	0.001
Peritumoral events	54	9	0.94 ± 0.26	0.42 to 1.46	0.001

^aSingle-slide method. χ² test of the null hypothesis that agreement between methods is no greater than expected by random chance.

^bNumber of invasive events by triple-stain minus number of invasive events by H and E, per patient.

^cPaired t-test of the null hypothesis that paired difference means = 0.