Table 4.

Sensitivity and specificity of tested methods to predict presence of metastasis in the lymph nodes by the presence of angio/lymphatic vessel invasion in the primary tumor. Standard pathological method using H and E, H and E on a single slide, and triple-stain immunohistochemistry on a single slide

	Angio/lymphatic vessel invasion
	Positive	Negative	Total	Case classificationa
Standard H and E
N+	7	20	27	Sensitivity 7/27 (25.9%)
N0	0	21	21	Specificity 21/21 (100%)
Total	7	41	48	Accuracy 28/48 (58.3%)
Single-slide H and E
N+	6	21	27	Sensitivity 6/27 (22.2%)
N0	3	18	21	Specificity 18/21 (85.7%)
Total	9	39	48	Accuracy 24/48 (50.0%)
Single-slide triple-stain
N+	22	5	27	Sensitivity 22/27 (81.5%)
N0	11	10	21	Specificity 10/21 (47.6%)
Total	33	15	48	Accuracy 32/48 (66.7%)

^aCase classification assumes pathological N stage is “truth” to which these methods are compared.