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Multiple sequence alignment to show the conservation of Ser-157 and Tyr-261 (FomC numbering) in all the clustered ADHs identified from phylogenetic analysis. The conserved serine and tyrosine are indicated by boxes. Pelobacter propionicus ADH, Staphylothermus marinus ADH, and Paracoccus denitrificans ADH belong to the group III metal-dependent ADH family, but are not clustered along with FomC, PhpC, and DhpG.
