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The N-terminal myristoyl group of FCaBP appears to be solvent-exposed. Two-dimensional (1H,13C HMQC) NMR spectra of free myristic acid dissolved in chloroform (A) and the myristoyl group of Ca2+-free (B) and Ca2+-bound (C) FCaBP. The myristoyl group was labeled with carbon-13, and the protein was unlabeled. The peaks in the spectrum represent protons attached to 13C-labeled fatty acyl chain.
