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Expression of the TbRGG2 protein in PF and BF life cycle stages of T. brucei. Proteins from either 1 × 106 or 1 × 107 total cells of PF strain 29-13 or BF single marker cells were resolved by 10% SDS-PAGE. The levels of the TbRGG2 protein were determined by Western blot with anti-TbRGG2 antiserum. Levels of Hsp70 were assessed as a loading control.
