FIGURE 5.

Co-immunoprecipitation of TbRGG2 with components of the editosome. Editosomes were immunoprecipitated from mitochondrial extract derived from PF 29-13 cells using a mix of monoclonal antibodies against KREPA1, KREPA2, KREPA3, and KREL1. For analysis of the unbound or bound immunoprecipitations, either 8% of the unbound fraction or 4% of the bead-bound fraction was analyzed by Western blotting (WB) for editosome components, MRP2, and TbRGG2. As a control, Protein A-Sepharose beads in the absence of antibody were also used for immunoprecipitation (Protein A IP). The asterisk indicates a nonspecific band that reacts with the anti-TbRGG2 polyclonal antibody in the Protein A-alone fractions.