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TbRGG2 is essential in both PF and BF T. brucei life cycle stages. The RNAi vector p2T7-177-TbRGG2 was transfected into both the PF strain 29-13 (A) and the BF single marker strain (B) of T. brucei. RNAi was induced by the addition of 2.5 μg/ml tetracycline to the cell media. Cells from duplicate cultures were counted every 2 days, and 1 × 106 cells were collected and immunoblotted for the presence of TbRGG2 or Hsp70.
