FIGURE 5.
Regulation of AC7 is mediated by the α subunit of G13. 293T cells expressing the CAMYEL sensor and either AC7 (A) or AC2 (B) were co-transfected with control DNA, or DNA encoding epitope-tagged Grk-PH or p115-rgRGS domains. Expression of the proteins was verified by Western blot using antibodies against the specific epitopes (C). 48 h post-transfection, cells were stimulated with 4 nm ISO at time 0 followed by 5 nm S1P at 120 s; cAMP responses were measured using the BRET assay. Error bars represent the standard deviation of results from three experiments. IB, immunoblot.