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. 2008 Aug 22;283(34):23200–23208. doi: 10.1074/jbc.M801146200

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FIGURE 2. — Activation of the mitochondrial pathway is required for the induction of apoptotic Top1cc by TRAIL. A, HCT116 cells of each genotype (Bax^+/- and Bax^-/-) were treated with 0.1 μg/ml TRAIL for the indicated times. Top panel, percentages of cells with apoptotic sub-G₁ DNA (means ± S.D. of triplicate samples). Bottom panel, detection of Top1cc. The DNA-containing fractions were pooled and probed at three concentrations (10, 3, and 1 μg) with an antibody against Top1. B and C, antimycin A (10 μm) and BH3I-2′ (30 μm) induce apoptotic Top1cc in Jurkat cells. Top panels, apoptotic DNA fragmentation (means ± S.D. of triplicate samples). Bottom panels, detection of Top1cc as for A.