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. 2008 Aug 22;283(34):23048–23061. doi: 10.1074/jbc.M800032200

FIGURE 4.

FIGURE 4.

Co-localization of PKCα with calnexin (A) or calreticulin (B) in control COS-1 cells. Cells were processed for co-immunofluorescence as described under “Experimental Procedures.” For calnexin and PKCα co-localization, calnexin was visualized with donkey anti-rabbit FITC conjugate, and PKCα was visualized with donkey anti-mouse TRITC conjugate. Assembly of fluorescence images (panels 1 and 2) indicates fusion-generated yellow immunofluorescence (panel 4) outside the nuclei (panel 5). For calreticulin and PKCα co-localization, calreticulin was detected with donkey anti-rabbit FITC conjugate, and PKCα was detected with donkey anti-mouse TRITC conjugate. Assembly of fluorescence images (panels 1 and 2) indicates fusion-generated yellow immunofluorescence (panel 4) outside the nuclei (panel 5). Co-localization of PKCα or PKCδ with UGT1A10His expressed in COS1 cells. Co-localization was determined by immunofluorescence that probed UGT1A10 with donkey anti-rabbit FITC conjugate and PKCα with donkey anti-mouse TRITC conjugate as described under “Experimental Procedures.” C, assembly of fluorescence images (panels 1 and 2) indicates fusion, which generated yellow immunofluorescence (panel 4) outside the nuclei (panel 5). D, co-localization of PKC δ and UGT1A10His expressed in COS-1. Co-localization was analyzed by immunofluorescence with PKCδ detected with donkey anti-rabbit FITC conjugate, and UGT1A10His was detected with donkey anti-mouse TRITC as described under “Experimental Procedures.” Assembly of fluorescence images (panels 1 and 2) merged to generate yellow fluorescence (panel 4) outside the nuclei as shown (panel 5). Background immunofluorescence images in non-transfected cells are shown in supplemental Fig. S4. Scale bars represent 20 μm(A) or 10 μm(B–D).