Mutant forms of eEF1A show enhanced thermostability compared with the
wild type protein. The wavelength spectrums of the 0.2–0.4 mg/ml
purified proteins are carried out at the indicated temperatures. A,
circular dichroism spectra of wild type eEF1A (• and ○) and A112T
(▴ and ▵) forms of eEF1A. The closed symbols represent the
wavelength spectrum at the native state (25 °C), whereas open
symbols represent the spectrum at 70 °C. B, circular
dichroism spectra of the mutant forms of eEF1A, R146K (○), T22S (▴),
A112T (▵), and A117V (▪), in comparison with wild type (•) at
70°C.