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Guanine nucleotides increase eEF1A stability. Circular dichroism spectra of apo-eEF1A (A), eEF1A with GDP (B), and eEF1A with GMPPNP (C) were measured. The spectra were deconvoluted into three basis curves using the convex constraint algorithm. Each curve (▪, ▵, and •) represents a different state of unfolding.
