GEF-independent mutants of eEF1A either affect the P-loop or the
NKXD element. A, Thr-22 (magenta) and Ala-112
(cyan) residues and their positions respective to the nucleotide
(green) and the P-loop (magenta) are shown. B,
Arg-164 (wheat) and Ala-117 (purple) residues and their
positions respective to the nucleotide (green) and the NKXD
motif (yellow) are shown. The structure was produced with the PyMOL
program (32), using the
co-crystal structure of the eEF1A·eEF1Bα complex with GDP
(Protein Data Bank code 1IJE)
(39). C, sequence
alignment of yeast eEF1A with the G-proteins independent of GEF in yeast
(eEF2, eIF5B, eRF3, Guf1p, and Hbs1p) and human (eEF2, eIF5B, eRF3, Guf1p,
Hbs1-like eRFs, and eEFSec). The alignment was generated by Jalview
(41).