TABLE 1.
Statistical characteristics of the 20 conformers of the solution structure of Dkk2C
| Parameter | |
|---|---|
| No. of NOE distance restraints | |
| Intraresidue | 448 |
| Interresidue | |
| Sequential | 561 |
| Medium range | 243 |
| Long range | 627 |
| Total | 1879 |
| No. of disulfides restraints | 30 |
| No. of Talos dihedral angle restraints | |
| π | 56 |
| ϰ | 56 |
| r.m.s. deviations from the mean (Å)a | |
| Overall structure,b backbone | 0.36 ± 0.11 |
| Gly177-Gln217 and Glu226-Ile259, backbone | 0.18 ± 0.04 |
| Overall structure,b heavy atoms | 0.82 ± 0.10 |
| Gly177-Gln217 and Glu226-Ile259, heavy atoms | 0.68 ± 0.10 |
| Residuesbin Ramachandran plot (%)c | |
| Most favorable regions | 84.7 |
| Additionally allowed regions | 13.4 |
| Generously allowed regions | 1.9 |
| Disallowed regions | 0.0 |
a
The average root mean square deviation (r.m.s.d.) between the 20 structures with the lowest target functions and the mean coordinates of the protein.
b
Residues Gly177-Ile259.
c
Excluding glycines and prolines and calculated using the Ramachandran macro in CYANA software.