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Localization studies of PLCβ and PI3K in HEK293 cells. (A) Images of HEK293 immunostained for PLCβ (left) and overexpressing p85α/eYFP-p110α (center), showing only negligible colocalization (right). (B) Example of a normalized FRET image from eCFP-PI3K to eYFP-PLCβ expressed in an HEK293 cell.
