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Ganglioside-mediated protection of FGF-1 and FGF-4 from tryptic digestion. Aliquots (1 μg) of native or heat-denatured (h.d.) FGF-2, FGF-1, or FGF-4 were incubated at 37°C for 3 h with 60 ng of trypsin in the absence or in the presence of 8 nmol of GT1b. Then samples were analyzed by 15% SDS-PAGE followed by silver staining of the gel.
