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. Author manuscript; available in PMC: 2008 Nov 13.
Published in final edited form as: Biochemistry. 2007 Oct 20;46(45):13101–13108. doi: 10.1021/bi701550c

Figure 2.

Figure 2

Reaction of wild type PTDH with protiated and deuterated phosphite in the steady state and pre-steady state. (A) Average of four pre-steady state traces in which a solution of 15 μM WT PTDH was mixed with an equal volume of a solution of 500 μM 1H or 2H phosphite and 500 μM NAD+ in 50 mM MOPS pH 7.25. (B) Phosphite concentration dependence of the steady state activity of 0.13 μM PTDH in the presence of 1 mM NAD+ in 100 mM MOPS pH 7.25. The lines represent fits to the Michaelis-Menten equation for protiated (circles) and deuterated phosphite (squares).