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. Author manuscript; available in PMC: 2008 Nov 13.
Published in final edited form as: Biochemistry. 2007 Oct 20;46(45):13101–13108. doi: 10.1021/bi701550c

Figure 5.

Figure 5

Reaction of representative active site mutants with protiated and deuterated phosphite in the pre-steady state. A) Reaction of R237K with protiated and deuterated phosphite in the pre-steady state. Reaction conditions: 100 mM MOPS pH 7.25, 100 mM 1H or 2H phosphite, 10 mM NAD+, 33 μM R237K. B) Reaction of E266Q with protiated and deuterated phosphite in the pre-steady state. Reaction conditions: 100 mM MOPS pH 7.25, 100 mM 1H or 2H phosphite, 5 mM NAD+, 15 μM E266Q. Each trace is the average of four separate shots. Background due to absorbance of NAD+ at 340 nm has been subtracted.