Figure 3. Differential antimicrobial effects on B. bronchiseptica for NO and H2O2 at LD50.
B. bronchiseptica were cultured on BG agar for 48 h following exposure of ~5,000 CFU to A) PBS, B) 250 µM SPER/NO, C) 5 µM H2O2, or D) 250 µM expired SPER/NO. The production of microcolonies was observed only after exposure to SPER/NO (B, white arrows). E) Microcolonies contained a significantly reduced number of CFU as compared to normal size colonies when grown on BG agar plates. Values are presented ± SEM. Results represent data from a total of 40 colonies collected from at least two independent experiments. “*” indicates statistical significance relative to all other samples (P < 0.05).