Table 2.
Name | Sequence a | % Response (n)b |
---|---|---|
Peptide 1c | EMPGVLRF-NH2 | 100 |
Peptide 2d | 29.1±5.7 (16) | |
Peptide 3 | 0 (3) | |
Peptide 4 | 19.0±2.6 (8) | |
Peptide 5 | SGSGAMPGVLRF-NH2 | 118.7±11.0 (4) |
Peptide 6 | AAAAAMPGVLRF-NH2 | 62.4±3.2 (10) |
Peptide 7e | 49.3±16.5 (4) | |
Peptide 8 | 0 (3) | |
Peptide 9f | 45.0±10.5 (6) | |
Peptide 10g | 104.8±2.8 (8) | |
Peptide 11 | PGVLRF-NH2 | 43.0±5.5 (14) |
Peptide 12 | PGVLRFPGVLRF-NH2 | 198.1±33.3 (10) |
Naturally occurring sequences are underlined. The conserved PGVLRF-NH2 sequence is in bold. N-terminal “extension” sequences of native long flp-18 peptides are bold and: Red for C. elegans based sequences and Blue for A. suum based sequences.
Peptides (10-6 M) were applied in 2 m pulses to Xenopus oocytes expressing NPR-1 215V. Results expressed as a % of response to 10-6 M Peptide 1 (EMPGVLRF-NH2) +/- SEM
Most active native C. elegans FLP-18 peptide
Longest and least active native FLP-18 peptide
Longest Ascaris AFP-1 peptide
Chimera of long FLP-18 + long AFP-1
Chimera of long AFP-1 + long FLP-1