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. Author manuscript; available in PMC: 2008 Aug 17.
Published in final edited form as: Biochemistry. 2006 Jun 20;45(24):7463–7473. doi: 10.1021/bi0602314

Fig.4.

Fig.4

UV cross-linking of 3H-AdoMet to Trm5 and mutants examined by SDS-PAGE analysis. (A) Commassie-stained of a representative SDS-PAGE analysis of the wild-type Trm5, and the R144A, Y176A, G205A/G207A, D223A, and P267A mutants. The intensity of the stain indicates the quantity of the enzyme in the gel. (B) The SDS-PAGE used in (A) subjected to fluorography imaging of the cross-linked species.