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Kinetic parameters with respect to AdoMet in the methyl transferase reaction
| kcat (min-1) | Km(μM) | kcat/Km (μM-1min-1) | Relative kcat/Km | |
|---|---|---|---|---|
| Wild-type Trm5 | 0.73 ± 0.01 | 1.0 ± 0.1 | 0.7 ± 0.1 | 1.0 |
| P267A | 0.006 ± 0.001 | 0.5 ± 0.04 | 0.01 ± 0.003 | 0.02 |
Kinetics of the wild-type Trm5 and P267A mutant was assayed with 0.2 μM enzyme, 6 μM tRNACys, and AdoMet ranging in 0.5-15 μM. The kcat and Km values are the average of at least 3 independent measurements.
