Table 3.
Determination of the equilibrium dissociation constant (Kd) for tRNA by gel shift
| WT | R144A | Y176A | G205A/G207A | D223A | P267A | |
| Kd (μM) | 0.4 ± 0.1 | 3.9 ± 0.6 | 1.7 ± 0.1 | 1.0 ± 0.4 | 4.6 ± 0.8 | 0.3 ± 0.1 |
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