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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2008 Apr 25;1782(7-8):462–468. doi: 10.1016/j.bbadis.2008.04.001

Figure 5. Nuclear co-localization of cdk5 and mutant G93A hSOD1 in muscle sections.

Figure 5

In vitro analysis of muscle samples revealed a mutant hSOD1 interaction-mediated reduction in cdk5 activity. Cdk5 and mutant hSOD1 localization were examined in muscle sections to determine whether the observed interactions are detectable in situ. (A) Nuclear staining of cdk5 is more widespread than that of mutant hSOD1. Only a subpopulation of cdk5 positive nuclei showed mutant hSOD1 immunostaining. Triple immunofluorescence shows distinct co-localization of cdk5 and mutant hSOD1 in the myonuclei. (B) Muscle fibers in G93A hSOD1 mice show aberrant nuclear patterning compared to those from Non-Tg mice. Nuclear patterning in the G93A hSOD1 mice muscle was clustered and disorganized in appearance compared to non-transgenic mice as shown by DAPI staining. Scale bar = 50 μm.