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ITC of TRAP. (a) Thermogram from a titration of tryptophan into TRAP (25 °C) showing the change in power required to maintain a constant temperature difference between the sample and reference cells upon injections of tryptophan. (b) Time-integrated heat data normalized per mole of injectant (squares) with nonlinear fit to identical and independent sites model (line). Best fit parameters are shown in Table 1.
