Fig. 1.
Overview of the strategy. Immunoprecipitation of hnRNPs from dormant/ non-tumorigenic cells allows for the identification of p38-target mRNAs (Subheading 3.1.). The upregulated messages may be knocked down via shRNA expressing vectors (Subheading 3.2.). The cells expressing shRNAs are introduced onto the chorioallantoinc membrame of a chick embryo (Subheading 3.3.). Growth of the shRNA cells above that of the parental cell line indicates that the knocked down gene is involved in tumor dormancy or survival (Subheading 3.4.). The other possible outcome (not shown, see Subheading 3.4.) is that the gene is required for survival and/or growth in vivo, so no tumor nodule will be detected.