Figure 1. Creatine enhances myonuclear addition in a dose-dependent manner.

A, representative images of primary mouse muscle cells differentiated for 48 h in the absence (–) or presence (+) of 75 mm creatine (CRT) are shown. Bar: 100 μm. B, a dose-dependent increase occurred in myonuclear number with creatine treatment for 48 h with evidence of saturation at higher concentrations. Data are means ±s.e.m. from three independent experiments. (*P < 0.05 relative to control.) C, primary myoblasts were treated with either 12.5 or 50 mm creatine. Creatine enhanced intracellular PCr accumulation as measured by HPLC analysis. All data were normalized to protein content and values are means ±s.e.m. from three independent experiments. (*P < 0.05 relative to control.)