Figure 2. Actin polymerization is required for creatine-mediated effects.

A, myoblasts were differentiated in the presence or absence of 12.5 mm creatine (CRT) for 24 h. This medium was subsequently replaced with DM containing vehicle, 50 or 100 nm latrunculin B (Lat B) for the remainder of myogenesis. Representative images following 48 h of differentiation are shown. Bar: 100 μm. B, myonuclear number was reduced to control levels in cells that received either 50 or 100 nm Lat B following creatine treatment. C, no significant difference in the total number of nuclei per field occurred with either creatine or Lat B treatment. Data are means ±s.e.m. from three independent experiments. (P < 0.05.)