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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: J Proteome Res. 2008 Jan 19;7(3):960–968. doi: 10.1021/pr070432+

Figure 1. Western Blot Analysis of Monkeypox IMV and EEV for an EEV-specific Protein.

Figure 1

Gradient purified monkeypox IMV and EEV particles were analyzed for the presence of MPV A35R protein, a homolog of the vaccinia EEV-specific 12 envelope glycoprotein A33R (MPV A35R shares 96.1% identity with VV A33R). Intact MPV particles were denatured in SDS-PAGE loading buffer and run in duplicate on 10% polyacrylamide gels. One of the gels was silver stained (left), and the replicate gel underwent Western blot analysis using a polyclonal antibody that recognizes VV A33R (right). MPV A35R is predicted to encode a 20 kDa protein, but was detected as a diffuse band at ∼45 kDa possibly because it is a glycoprotein or because it comigrated with other viral proteins 54. The numbers (left) indicate molecular masses in kDa.