. Author manuscript; available in PMC: 2009 Mar 1.

Published in final edited form as: J Proteome Res. 2008 Jan 19;7(3):960–968. doi: 10.1021/pr070432+

Table 1.

Summary of Peptide and Protein Identifications

Monkeypox Virus:		LC-MS/MS			LC-MS
Unique Peptide Identifications by:		Global	SCX	Total	Global
SEQUEST®, but not X! Tandem	unmodified	1,313	3,332	3,495	157 (4%)
SEQUEST® & X! Tandem	unmodified	6,749	12,062	13,970	7,154 (51%)
X! Tandem, but not SEQUEST® {	unmodified Modified^*	1,363 855	2,883 2,672	3,187 2,925	92 (3%) 657 (22%)
Total:		10,280	20,949	23,577	8,060 (34%)
Protein Identifications:		1,843	2,705	2,718	1,677 (62%)

Vaccinia Virus:		LC-MS/MS			LC-MS
Unique Peptide Identifications by:		Global	SCX	Total	Global
SEQUEST®, but not X! Tandem	unmodified	859	1,871	2,266	96 (4%)
SEQUEST®& X! Tandem	unmodified	3,124	7,876	9,355	3,053 (33%)
X! Tandem, but not SEQUEST® {	unmodified modified^*	540 439	1,524 1,852	1,683 2,080	20 (1%) 353 (17%)
Total:		4,962	13,123	15,384	3,522 (23%)
Protein Identifications:		962	1,814	1,847	747 (40%)

Met oxidation, amino-terminal acetylation, Gln NH₃ loss, or Glu H₂O loss