Figure 4.

Western blots with a superoxide dismutase (SOD-1) antibody of rat urine samples collected during the induction of liver fibrosis with carbon tetrachloride (CCl₄) repeatedly dosed at 0.7 ml/kg. Samples for sodium dodecyl sulphate–polyacrylamide gel electrophoresis were prepared, with each sample containing 14 μl of urine. (a) Urine collected after 3 weeks of dosing twice a week with CCl₄. (b) Urine collected after 6 weeks of dosing twice a week with CCl₄. (c) Urine collected at the end of the 6-week non-dosing recovery period. In all blots: lane 1, 2, 3 = control urine from rats treated with vehicle, and lanes 4 and 5 = urine from rats treated with CCl₄ at 0.7 ml/kg.