Table 2.
Mean (±SD) of serum clinical chemistry parameters of female Hanover Wistar rats treated with carbon tetrachloride (CCl4) at dose levels from 0.4 to 1.0 ml/kg twice weekly for 6 weeks†
| Serum chemistry parameter | Dose of CCl4 (ml/kg) | |||||||
|---|---|---|---|---|---|---|---|---|
| 0 (control) | 0.4 | 0.5 | 0.6 | 0.7 | 0.8 | 0.9 | 1.0 | |
| ALT | 39.50 ± 9.35 | 225.67 ± 222.97 | 576.17* ± 416.95 | 502.40*** ± 158.41 | 1088.67 ± 1280.35 | 995.83* ± 770.51 | 789.83* ± 705.70 | 1544.33** ± 948.40 |
| AST | 123.17 ± 15.20 | 325.50* ± 192.29 | 704.17* ± 590.27 | 557.20** ± 228.61 | 1095.17 ± 1316.29 | 1019.33* ± 724.55 | 867.50* ± 805.10 | 1912.33* ± 1509.21 |
| GLDH | 4.63 ± 0.98 | 110.65 ± 145.51 | 296.53 ± 409.14 | 279.26* ± 213.69 | 714.38 ± 1140.30 | 502.20* ± 444.16 | 433.07* ± 387.32 | 722.25* ± 574.37 |
| LDH | 3035.67 ± 841.80 | 2380.33 ± 687.20 | 3927.50 ± 2133.18 | 2945.00 ± 2006.42 | 3448.50 ± 2286.28 | 1944.67* ± 622.36 | 1825.17 ± 111.63 | 2644.17 ± 969.37 |
| CK | 1150.33 ± 304.01 | 830.50 ± 237.27 | 1026.67 ± 531.30 | 658.40* ± 293.53 | 660.00* ± 356.85 | 573.83** ± 204.11 | 575.67* ± 342.04 | 468.83*** ± 134.72 |
| ALP | 204.67 ± 28.19 | 316.33* ± 103.57 | 393.83*** ± 60.17 | 421.20*** ± 77.55 | 676.17* ± 411.27 | 592.00*** ± 167.64 | 526.00** ± 205.41 | 555.83* ± 281.13 |
| T-BIL | 3.28 ± 0.17 | 4.73** ± 0.92 | 4.40* ± 0.98 | 4.94*** ± 0.72 | 7.05 ± 4.44 | 5.08 ± 2.35 | 5.33*** ± 0.73 | 6.23* ± 2.40 |
| BA | 35.28 ± 15.06 | 84.68*** ± 18.36 | 128.60** ± 56.31 | 148.24** ± 56.91 | 127.28** ± 55.82 | 133.67** ± 60.17 | 110.28** ± 44.81 | 135.60** ± 57.47 |
| ALB | 38.02 ± 0.97 | 36.77 ± 1.72 | 35.62* ± 1.75 | 36.80 ± 1.04 | 35.67* ± 1.74 | 35.18*** ± 0.93 | 36.43 ± 1.65 | 34.80* ± 2.57 |
| UREA | 7.43 ± 0.37 | 9.63** ± 1.17 | 9.79*** ± 0.91 | 9.84*** ± 0.80 | 9.42** ± 1.08 | 9.24* ± 1.40 | 9.19** ± 1.17 | 9.15*** ± 0.78 |
| CREA | 57.83 ± 2.71 | 56.67 ± 1.97 | 56.50 ± 3.94 | 54.50* ± 0.89 | 51.17* ± 4.45 | 52.17* ± 3.54 | 56.17 ± 3.43 | 51.67* ± 5.13 |
| SOD | 386.67 ± 55.72 | 593.50* ± 179.42 | 772.50*** ± 175.77 | 739.20*** ± 34.10 | 612.33* ± 237.89 | 701.67** ± 170.36 | 760.50*** ± 147.87 | 726.50*** ± 98.64 |
| TP | 64.42 ± 2.20 | 60.38** ± 1.01 | 59.18** ± 1.96 | 61.22* ± 1.63 | 58.37** ± 2.76 | 57.83*** ± 2.44 | 55.63 ± 14.21 | 57.33** ± 4.45 |
| Ca | 2.52 ± 0.05 | 2.51 ± 0.08 | 2.53 ± 0.08 | 2.54 ± 0.10 | 2.52 ± 0.10 | 2.47 ± 0.12 | 2.52 ± 0.09 | 2.54 ± 0.15 |
| CHOL | 1.56 ± 0.25 | 1.24* ± 0.23 | 1.16* ± 0.20 | 1.14** ± 0.14 | 1.35 ± 0.40 | 1.30 ± 0.21 | 1.28* ± 0.09 | 1.35 ± 0.46 |
| TRIG | 0.79 ± 0.27 | 0.52 ± 0.29 | 0.38** ± 0.11 | 0.29** ± 0.06 | 0.65 ± 0.58 | 0.35** ± 0.04 | 0.31** ± 0.04 | 0.34** ± 0.08 |
| GLU | 8.42 ± 0.81 | 6.78** ± 0.66 | 5.69*** ± 0.52 | 6.25*** ± 0.45 | 6.02** ± 1.50 | 5.87*** ± 0.73 | 6.31*** ± 0.74 | 5.40*** ± 0.89 |
ALT, alanine aminotransferase (U/l); AST, asparate aminotransferase (U/l); GLDH, glutamate dehydrogenase (U/l); LDH, lactate dehydrogenase (U/l); CK, creatine kinase (U/l); ALP, alkaline phosphatase (U/l); T-BIL, total bilirubin (μmol/l); BA, bile acids (mmol/l); ALB, serum albumin (g/l); Urea (mmol/l); CREA, creatinine (μmol/l); SOD, superoxide dismutase (U/l); TP, total protein (g/l); Ca, calcium (mmol/l); CHOL, cholesterol (mmol/l); TRIG, triglyerides (mmol/l); GLU, glucose (mmol/l).
Rats were treated with CCl4 by gavage.
Significantly different from control, P < 0.05,
P < 0.01,
P < 0.001. n = 6 rats per dose level group.