Table 3.
Mean (±SD) of serum clinical chemistry parameters of female Hanover Wistar rats treated with carbon tetrachloride (CCl4) at dose levels from 0.4 to 1.0 ml/kg twice weekly for 6 weeks followed by a 6-week recovery period without dosing (week 12)†
| Serum chemistry parameter | Dose of CCl4 (ml/kg) | |||||||
|---|---|---|---|---|---|---|---|---|
| 0 (control) | 0.4 | 0.5 | 0.6 | 0.7 | 0.8 | 0.9 | 1.0 | |
| ALT | 45.50 ± 7.53 | 43.33 ± 9.69 | 43.67 ± 8.59 | 56.17 ± 26.42 | 41.67 ± 8.14 | 35.67 ± 11.33 | 40.00 ± 7.38 | 42.00 ± 7.01 |
| AST | 144.50 ± 14.01 | 133.67 ± 18.03 | 134.00 ± 18.89 | 126.67 ± 26.61 | 117.17** ± 15.33 | 99.67* ± 37.53 | 102.33*** ± 10.33 | 123.50* ± 14.63 |
| GLDH | 5.97 ± 6.60 | 4.60 ± 4.95 | 2.88 ± 0.69 | 4.13 ± 2.92 | 2.13 ± 1.03 | 2.02 ± 1.24 | 3.03 ± 2.63 | 6.17 ± 7.80 |
| LDH | 3298.83 ± 242.74 | 2896.33 ± 492.15 | 2939.83 ± 725.70 | 2578.67** ± 343.31 | 2506.33** ± 347.69 | 1849.17** ± 781.07 | 1759.83*** ± 633.69 | 2217.83* ± 905.5 |
| CK | 1416.83 ± 130.17 | 1225.83 ± 202.97 | 1233.17 ± 321.34 | 1046.33** ± 153.52 | 959.33*** ± 125.70 | 775.00*** ± 316.59 | 720.17*** ± 233.58 | 795.50*** ± 240.55 |
| ALP | 65.33 ± 5.92 | 66.67 ± 4.37 | 65.67 ± 6.35 | 69.17 ± 25.10 | 68.83 ± 20.85 | 75.83 ± 27.55 | 77.33 ± 28.39 | 76.83 ± 8.26 |
| T-BIL | 3.68 ± 0.36 | 3.37 ± 0.27 | 3.77 ± 0.36 | 3.97 ± 0.39 | 4.33 ± 0.65 | 4.25 ± 1.37 | 4.28 ± 0.57 | 3.87 ± 0.69 |
| BA | 29.65 ± 6.04 | 41.67 ± 16.57 | 51.18* ± 19.84 | 54.12* ± 23.95 | 42.45* ± 8.96 | 34.35 ± 10.92 | 49.27 ± 21.61 | 50.07* ± 19.49 |
| ALB | 38.13 ± 1.77 | 37.50 ± 1.44 | 36.88 ± 1.64 | 38.47 ± 1.54 | 38.03 ± 1.47 | 37.22 ± 1.24 | 36.55 ± 1.26 | 35.63* ± 1.13 |
| UREA | 7.48 ± 0.36 | 8.17** ± 0.24 | 8.65 ± 1.38 | 9.09** ± 1.13 | 7.20 ± 1.36 | 6.80 ± 1.37 | 6.93 ± 0.87 | 6.40* ± 1.02 |
| CREA | 57.50 ± 13.17 | 63.33 ± 5.79 | 63.83 ± 4.54 | 64.50 ± 3.27 | 60.67 ± 5.43 | 55.67 ± 11.47 | 56.00 ± 3.74 | 58.17 ± 3.76 |
| SOD | 622.67 ± 139.03 | 513.83 ± 113.99 | 444.33 ± 143.27 | 494.50 ± 201.06 | 471.67* ± 48.50 | 473.17* ± 76.49 | 455.17* ± 85.92 | 616.67 ± 113.05 |
| TP | 66.05 ± 2.69 | 64.05 ± 2.35 | 63.80 ± 3.33 | 65.73 ± 2.72 | 66.75 ± 2.70 | 63.63 ± 2.54 | 64.07 ± 1.93 | 62.92 ± 3.41 |
| Ca | 2.45 ± 0.08 | 2.39 ± 0.07 | 2.42 ± 0.10 | 2.44 ± 0.03 | 2.46 ± 0.09 | 2.45 ± 0.06 | 2.47 ± 0.08 | 2.44 ± 0.06 |
| CHOL | 1.03 ± 0.22 | 1.69** ± 0.34 | 1.56* ± 0.40 | 1.52** ± 0.29 | 1.62** ± 0.26 | 1.65*** ± 0.13 | 1.78*** ± 0.19 | 1.19 ± 0.20 |
| TRIG | 0.56 ± 0.19 | 0.52 ± 0.23 | 0.78 ± 0.40 | 0.66 ± 0.39 | 0.44 ± 0.07 | 0.47 ± 0.23 | 0.60 ± 0.49 | 0.43 ± 0.10 |
| GLU | 4.68 ± 2.00 | 3.85 ± 1.21 | 4.31 ± 1.26 | 4.52 ± 2.09 | 5.36 ± 1.08 | 4.43 ± 1.44 | 4.48 ± 1.01 | 4.54 ± 1.23 |
ALT, alanine aminotransferase (U/l); AST, asparate aminotransferase (U/l); GLDH, glutamate dehydrogenase (U/l); LDH, lactate dehydrogenase (U/l); CK, creatine kinase (U/l); ALP, alkaline phosphatase (U/l); T-BIL, total bilirubin (μmol/l); BA, bile acids (mmol/l); ALB, serum albumin (g/l); Urea (mmol/l); CREA, creatinine (μmol/l); SOD, superoxide dismutase (U/l); TP, total protein (g/l); Ca, calcium (mmol/l); CHOL, cholesterol (mmol/l); TRIG, triglyerides (mmol/l); GLU, glucose (mmol/l).
Rats were treated with CCl4 by gavage.
Significantly different from control, P < 0.05,
P < 0.01,
P < 0.001. n = 6 rats per dose level group.