Figure 2.

Western blot analysis of carboxypeptidase, vitellogenic-like (CPVL) expression in human immune cells. (a) CPVL is expressed by monocytes (MO), monocyte-derived macrophages (MDM) and PMA-stimulated THP-1 cells, but not by monocyte-depleted peripheral blood mononuclear cells (MO^-PBMC). Protein levels are higher in MDM than in MO. (b) CPVL expression in monocyte-derived dendritic cells (MoDC) is unaffected by treatment with LPS for up to 48 h and is comparable with that in MDM. (c) CPVL in MDM is unaffected by treatment with O-glycosidase. (d) Treatment with N-glycosidase or endoglycosidase H (endo H) lowers the CPVL band in MDM. (e) CPVL in THP-1 and Jurkat cell lines are insensitive to treatment with a cocktail of O-glycosidase, N-glycosidase and endo H (glyco). In all experiments, protein loading for all samples was equal.