. 2006 Feb;87(1):49–63. doi: 10.1111/j.0959-9673.2006.00455.x

Table 2.

Haematological results from female BALB/c mice treated with busulphan (BU) at 8.25, 9.0 and 9.75 mg/kg on 10 occasions over a period of 21 days and sampled at days 1, 23, 42, 71, 84, 106 and 127 after the final dose

Autopsy (days post-dosing)	BU group (mg/kg)	RBC	Hb	MCV	MCH	Retic	WBC	Neut	Lymph	Mono	Plt	FNCC	Spleen	n
1	Control	10.77	16.0	46.6	14.8	257.7	2.7	0.54	1.99	0.07	811	12.96	5548	9
	8.25	10.06^**	15.4	48.3	15.3	237.9	2.0^*	0.15^***	1.76	0.02^***	138^***	6.20^***	4766^**	7
	9.0	6.97^**	10.8^*	51.3^*	15.8^*	158.9^**	1.0^***	0.09^***	0.87^**	0.01^***	107^***	3.57^***	4798^*	7
	9.75	8.22^**	13.0^*	50.1^*	16.0^***	244.1	1.4^***	0.11^***	1.27^**	0.02^***	135^***	4.93^***	4461^*	7
23	Control	10.66	15.9	48.6	14.9	307.1	1.7	0.34	1.41	0.04	881	10.11	5044	7
	8.25	10.17^*	16.6^**	53.5^***	16.4^***	282.5	1.5	0.32	1.05^*	0.04	474^***	6.19^***	4445	7
	9.0	8.98^***	14.4^*	52.8	16.1	407.1	1.6	0.59	0.95^*	0.02	430^**	4.94^**	7293	7
	9.75	9.45^**	14.9^**	52.3^*	15.9^*	251.4	2.2	0.83^*	1.25	0.04	629^*	6.76^*	5871	7
42	Control	10.25	15.6	49.8	15.3	224.4	1.9	0.40	1.24	0.06	797	10.79	5195	7
	8.25	9.76^*	15.3	51.2^*	15.8^**	285.8^*	1.3^*	0.25^**	0.97^*	0.04	599^***	8.83^*	4484^*	7
	9.0	9.44^***	15.3	51.7^**	16.2^**	266.6	0.9^***	0.22^***	0.61^***	0.02^*	610^***	8.40^*	4225^*	7
	9.75	10.01	15.7	50.7^*	15.7^**	230.1	0.9^***	0.21^**	0.63^***	0.03	645^***	9.11	4558^*	7
71	Control	10.55	15.5	47.4	14.7	309.6	1.7	0.29	1.20	0.05	934	11.94	4521	7
	8.25	10.40	15.8	49.1^**	15.2^*	257.0^*	1.5	0.31	1.08	0.04	568^**	10.19^*	4012^*	7
	9.0	9.77^***	14.8^**	48.6^*	15.1^*	287.0	1.2	0.24	0.82	0.03	589^**	8.66^**	4217	7
	9.75	10.05^**	15.4	48.8^*	15.3^**	222.1^**	1.2^*	0.23	0.84^*	0.03	553^***	9.30^***	4328	7
84	Control	10.31	15.3	47.7	14.8	270.7	1.9	0.40	1.27	0.08	932	15.24	4491	8
	8.25	10.21	15.4	49.0^***	15.1^***	267.8	1.7	0.34	1.18	0.04^**	655^***	11.49^*	3896^***	8
	9.0	9.99^*	15.2	50.2^***	15.2^***	282.2	1.2^*	0.35	0.77^*	0.03^**	691^***	10.49^*	3977^**	8
	9.75	9.73^*	14.9	50.5^**	15.4^*	271.6	1.4	0.32	0.92	0.05	566^***	11.29	4368	8
106	Control	10.39	15.7	47.9	15.1	250.7	1.4	0.29	0.93	0.05	935	13.13	4376	10
	8.25	10.28	15.8	48.5^*	15.4^**	271.2	2.4^**	0.52^**	1.59^**	0.07	731^***	9.26^***	3614^***	10
	9.0	9.31^**	14.7^*	50.7^**	15.7^*	286.5	1.0	0.26	0.62^*	0.03	598^***	8.45^***	4250	10
	9.75	9.94^**	15.5	48.9^*	15.7^***	238.1	1.2	0.34	0.74	0.04	643^***	9.70^*	3981^*	10
127	Control	10.24	15.5	48.4	15.1	240.7	1.4	0.31	0.99	0.05	938	13.81	4086	15
	8.25	9.91^*	15.2	49.0	15.4	272.4^*	1.5	0.39	0.96	0.05	658^***	10.36^***	3695^*	8
	9.0	9.77^***	15.3	50.1^***	15.6^***	239.6	1.4	0.43^*	0.90	0.05	699^***	9.39^***	3520^***	15
	9.75	9.85^*	15.3	49.3	15.3^*	239.7	1.3	0.38	0.76	0.04^*	599^***	9.65^***	3607^**	11

P < 0.05.

^**

P < 0.01.

^***

P < 0.001.

Values are means; P-values are significantly different to control animals.

Abbreviations and units: RBC, red blood cells (×10⁶/µl); Hb, haemoglobin (g/dl); MCV, mean cell volume (fl); MCH, mean cell haemoglobin (pg); Retic, absolute reticulocyte count (×10³/µl); WBC, white blood cells (×10³/µl); Neut, neutrophils (×10³/µl); Lymph, lymphocytes (×10³/µl); Mono, monocytes (×10³/µl); Plt, platelets (×10³/µl); FNCC, femoral nucleated cell count (×10⁶); Spleen, relative spleen weight (mg/kg body weight); n, number of mice per group. At day 127, lymphoma-bearing mice (n = 5) were not included in the analysis of the 8.25 and 9.0 mg/kg BU group results; blood samples from two mice sampled at day 127 (8.25 mg/kg BU) could not be analysed due to the presence of clots.