Table 1.
Mean erythroid parameters in female B6C3F1 mice dosed with chloramphenicol succinate (CAPS) at 2500 and 3500 mg/kg on five consecutive days and sampled at 1, 7, 14 and 21 days after the final dose
| CAPS dose (mg/kg) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Control | 2500 | 3500 | ||||||||||
| Day post-dosing | 1 | 7 | 14 | 21 | 1 | 7 | 14 | 21 | 1 | 7 | 14 | 21 |
| RBC | 9.848 | 9.770 | 9.688 | 9.900 | 8.855** | 8.058** | 9.682 | 10.043 | 8.968** | 7.642** | 9.955 | 9.658 |
| HCT | 0.542 | 0.538 | 0.533 | 0.531 | 0.480** | 0.448** | 0.535 | 0.546 | 0.487** | 0.411** | 0.545 | 0.512 |
| Hb | 15.45 | 15.38 | 15.13 | 15.36 | 13.93** | 12.42** | 15.24 | 15.58 | 14.16** | 11.70** | 15.53 | 14.86 |
| MCV | 55.05 | 55.06 | 55.00 | 53.60 | 54.23 | 55.58 | 55.30 | 54.35 | 54.32 | 53.78** | 54.75 | 52.98 |
| MCH | 15.70 | 15.74 | 15.60 | 15.52 | 15.70 | 15.42 | 15.74 | 15.50 | 15.78 | 15.32* | 15.60 | 15.38 |
| MCHC | 28.53 | 28.60 | 28.35 | 28.94 | 28.98 | 27.74 | 28.42 | 28.58 | 29.02 | 28.50 | 28.48 | 29.04 |
| RDW | 11.60 | 11.84 | 11.85 | 11.66 | 11.10** | 12.70** | 12.86** | 12.71** | 10.92** | 11.32 | 13.80** | 13.26** |
| HDW | 1.500 | 1.514 | 1.558 | 1.492 | 1.430* | 1.550** | 1.622** | 1.660** | 1.392* | 1.410** | 1.665** | 1.708** |
| Retic | 318.4 | 274.1 | 280.9 | 249.4 | 6.0** | 414.1 | 275.4 | 249.2 | 6.1*** | 19.5*** | 397.8 | 212.6 |
| HFR | 77.5 | 54.1 | 49.6 | 39.4 | 0.1** | 170.8** | 50.8 | 39.2 | 0.2*** | 1.5*** | 77.1 | 28.3 |
| BM cells | 1.180 | 1.258 | 1.053 | 1.106 | 0.960 | 1.588 | 1.073 | 1.398 | 1.050 | 1.196 | 0.983 | 1.378 |
| BM eryth cells | 0.423 | 0.380 | 0.253 | 0.300 | 0.088*** | 0.558 | 0.345 | 0.400 | 0.040*** | 0.260 | 0.305 | 0.326 |
| BFU-E | 42.67 | nd | 45.33 | 30.00 | 33.67** | nd | 46.00 | 25.00* | 26.33*** | nd | 46.33 | 28.33 |
| CFU-E | 47.00 | nd | 48.67 | 60.00 | 35.00* | nd | 49.67 | 60.00 | 33.33* | nd | 39.00 | 58.67 |
| Splenic cells | 8.68 | 8.82 | 9.82 | 9.91 | 5.01*** | 17.44*** | 11.94 | 11.86 | 3.73*** | 8.31 | 11.65 | 10.59 |
| Splenic eryth | 3.2 | 1.8 | 1.8 | 2.0 | 0.0 | 4.0 | 1.6 | 2.0 | 0.0 | 2.6 | 3.0 | 2.2 |
| Plt | 847.3 | 970.4 | 1072.5 | 987.0 | 897.8 | 1261.8* | 1254.0 | 1084.0 | 770.4 | 1765.0* | 1066.0 | 973.0 |
Abbreviations and units: RBC, red blood cells (×1012/l); HCT, haematocrit (l/l); Hb, haemoglobin (g/dl); MCV, mean cell volume (fl); MCH, mean cell haemoglobin (pg); MCHC, mean cell haemoglobin concentration (g/dl); RDW, red cell (volume) distribution width femoral (%); HDW, haemoglobin (cellular concentration) distribution width (g/dl); Retic, absolute reticulocyte count (×109/l); HFR, high fluorescence ratio reticulocytes (×109/l); BM cells, bone marrow nucleated cell count (×107); BM eryth cells, femoral bone marrow erythroid cells (×107); BFU-E, burst-forming units-erythroid, absolute number of colonies per plate; CFU-E, colony-forming units-erythroid, absolute number of colonies per plate; Splenic cells, spleen suspension nucleated cell count (×107); Splenic eryth, splenic erythropoietic grading, histological grading: 0, absent; 1, very slight; 2, slight; 3, moderate; 4, marked; Plt, platelets, (×109/l); nd, not determined. n = 5 for control and CAPS groups at all time points, except n = 4 for 3500 mg/kg CAPS group at day 14 and for BFU-E and CFU-E where n = 3.
Significantly different from control animals
P < 0.05
P < 0.01
P < 0.001. Data for splenic erythropoietic grading was not analysed statistically.