Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Real-time PCR primer sequences
| Gene | Upstream primer | Downstream primer | Size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| G3PDH | GAAGGTGAAGGTCGGAGT | TGGCAACAATATCCACTTTACCA | 92 | 60 |
| Smad3 | ATCTACTGCCGCCTGTGG | TCTCTGGTAGTGGTAGGGATTC | 129 | 64 |
| Smad4 | GCCAACTTTCCCAACATTCCTG | TGCTGCTGTCCTGGCTGAG | 116 | 68 |
| Smad5 | CTTTCCACCAGCCCAACAACAC | TAGGCAGGAGGAGGCGTATCAG | 151 | 68 |
| BMP-7 | GACGCTGGTCCACTTCATCAAC | TGGAGCTGTCATCGAAGTAGAGG | 101 | 60 |
