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Double-label immunofluorescence staining in cultured endothelial cells of hypoxic group (in the same field) Hoechst fluorescence showing cell nuclei (left), SMA expression is negative in the cytoplasm by day 1 (top, right); SMA is expressed in a cytoplasmic pattern by days 7, and the SMA-positive cells alter their morphological appearance toward an elongated, spindle-shaped form (bottom, right).
