Figure 4.

Immunohistochemistry. Representative views of the prestenotic segments of the aorta from operated rats and the corresponding segments from controls. The arrow read (brown stained features) point out the expression of the endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS), nitrotyrosine and transforming growth factor-β (TGF-β) in operated animals. The graphs represent the quali-quantitative evaluation of the immunohistochemical analysis revealing an increased expression of eNOS, iNOS, nitrotyrosine and TGFβ in the prestenotic segment in comparison with that of corresponding segments from control aortas. (□, Sham-operated; ▪, operated). Scale bars, 20 μm.