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Morphology and immunofluorescence of CAC2 cells grown on plain substrate (glass). Phase contrast shows a monolayer of polyedrical cells. Nuclei are round, with two to three nucleoli, and the cytoplasm are abundant (a). Immunofluorescence exhibits cytokeratin, smooth-muscle actin and vimentin (b–d). (original magnification a, 200×; b–d, 400×).
