Figure 4.

Release of amyloid-β (Aβ) from the aggregate with apolipoprotein E (apoE) in Alzheimer's disease (AD) brains. Immunoprecipitation with the apoE antibody AB947 and immunodetection with the monoclonal anti-apoE Mab1062 show in the range of 34–40 kDa broad band corresponding to an apoE/Aβ42 complex that is under-represented in AD subjects in comparison with controls (CTR). The band corresponding to apoE/Aβ42 is visible in the subject homozygous ε4 only after a longer exposure. The lower portion of the gel, immunostained with the monoclonal anti-Aβ antibody 6E10, reveals the typical Aβ pattern for AD cases, indicating that a portion of Aβ peptides is released from the complex with apoE after SDS/PAGE, only in AD samples.