Table 1.

Effect of coculturing, tumour cell-conditioned media and growth factors on extracellular matrix (ECM) component production on microscopic slides and in three-dimensional gels

		ECM components
		CS		C-6-S		Tenascin-C		Versican
Cells	Culture conditions	M	G	M	G	M	G	M	G
HT-29	10% FCS	−	−	−	−	+	+	−	−
	NFCS or 1% FCS	−	−	−	−	+	+	−	−
	S-CM	−	−	−	−	+++	++	−	−
	TGF-β	−	−	−	−	+++	+++	−	−
Caco-2	10% FCS	−	−	−	−	−	−	−	−
	NFCS or 1% FCS	−	−	−	−	−	−	−	−
	S-CM	−	−	−	−	++	++	−	−
	TGF-β	−	−	−	−	+++	+++	−	−
Stromal cells	10% FCS	+	+	+	+	+	+	+	+
	NFCS or 1% FCS	+	+	+	+	+	+	+	+
	Caco-2-CM	+++	++	+++	++	+++	++	+++	++
	Caco-2-CM + anti-TGF-β	+	+	+	+	+	+	+	+
	HT-29-CM	++	++	++	++	+	++	++	+++
	HT-29-CM + anti-TGF-β	+	+	+	+	+	+	+	++
	TGF-β	+++	+++	++	++	+++	+++	+++	++
	PDGF	+++	+++	+++	+++	+	+	+++	+++
	bEGF	+	+	+	+	+	+	+	+
	IGF-I	+	+	+	+	+	+	+	+
	IGF-II	+	+	+	+	+	+	+	+
Caco-2 and stromal cells	NFCS or 1% FCS	+++	+++	+++	+++	+++	+++	+++	++
HT-29 and stromal cells	NFCS or 1% FCS	+++	+++	+++	+++	+++	+++	+++	+++

FCS, fetal calf serum; NFCS, no fetal calf serum; S-CM, stromal cell-conditioned media; Caco-2-CM, Caco-2 cell-conditioned media; HT-29-CM, HT-29-conditioned media; TGF, transforming growth factor; PDGF, platelet-derived growth factor; bEGF, basic epidermal growth factor; IGF, insulin-like growth factor; M, monolayers on plastic; G, three-dimensional gels; +++, strong reaction; ++, moderate reaction; +, weak reaction; –, negative reaction.