Figure 4.

Non-structural protein-1 (NS1) can be identified only in dengue virus (DV)-inoculated explants. Tissue sections obtained from the skin explants under different treatments were all incubated with a murine monoclonal antibody to recombinant NS1 from DV. Positivity was developed with peroxidase to obtain blue dark colour, while counterstaining was performed with nuclear fast red, providing a reddish staining. (a) illustrates sections from freshly prepared skin, (b) skin sections from controlcultured explants, (c) skin from mock-infected explants and (d) the blue dark labelling obtained in explants inoculated with DV. Pictures are representative of three different subjects assessed.