Table 2.
Details of the clones, dilutions and suppliers of the primary mouse IgG1 monoclonal antibodies used
| Primary monoclonal antibody | Clone | Dilution | Supplier |
|---|---|---|---|
| Melan A | A103 | 1:25 | Novocastra (Newcastle-upon-Tyne, UK) |
| S100 | S1/61/69 | 1:30 | Novocastra (Newcastle-upon-Tyne, UK) |
| HMB45 | HMB45 | 1:45 | Novocastra (Newcastle-upon-Tyne, UK) |
| Tyrosinase | T311 | 1:25 | Novocastra (Newcastle-upon-Tyne, UK) |
| CD1a | JPM30 | 1:20 | Novocastra (Newcastle-upon-Tyne, UK) |
| CD45 | X16/99 | 1:15 | Novocastra (Newcastle-upon-Tyne, UK) |
| CD68 | KP1 | 1:100 | Dako Corporation (Carpinteria, CA, USA) |
| CD68 | PG-M1 | 1:100 | Dako Corporation (Carpinteria, CA, USA) |
| CD163 | 10D6 | 1:50 | Novocastra (Newcastle-upon-Tyne, UK) |