Table 4.

Results of staining uveal melanoma sections for the macrophage markers CD68 and CD163, and the leucocyte antigen CD45, and approximate levels of infiltration as assessed by Dr h. S. Mudhar

			Intensity of staining
SOM	Percentage of infiltrating cells	Cell type	CD45	CD68 (PG-M1)	CD163
157d	<1	Macrophages		+++	+/++
	10	Leucocytes	++
196B	<1	Macrophages		+++	++
	0	Leucocytes	–
256	<1	Macrophages		+++	++
	0	Leucocytes	–
258	0	Macrophages		–	–
	0	Leucocytes	–
267	<1	Macrophages		++	++
	<1	Leucocytes	++
280	<1	Macrophages		++	++
	0	Leucocytes	–
330	<1	Macrophages		++	++
	0	Leucocytes	–
346	<1	Macrophages		++	++
	0	Leucocytes	–
349	<1	Macrophages		+++	–/+
	0	Leucocytes	–
350	<1	Macrophages		+++	+++
	<1	Leucocytes	+++
351 met	<1	Macrophages		+++	+++
	0	Leucocytes	–

Levels of infiltration are expressed as an approximate percentage of the total cell population. The anti-CD45 monoclonal antibody detects a range of leucocyte cell types, but morphologically immunopositive cells resembled lymphocytes. Cells expressing the dendritic cell marker CD1a were absent from all sections. Staining intensity was assessed as described for Table 3. Double-labelling immunofluorescence identified that infiltrating macrophages were S100-negative, whereas melanoma cells commonly expressed both S100 and HMB45.