Figure 5.

Loss of vinculin from focal adhesions during [Ca²⁺]-induced integrin redistribution. VPMs from adherent CEFs were fixed after preparation on ice (A and D) or after incubation for 15 min at 37°C in HCB (B and E) or in LCB (C and F). VPMs were then processed for immunofluorescence using the β1-cyto polyclonal antibody (A–C) and the anti-vinculin mAb (D–F). The same fields are shown in A and D, B and E, and C and F. Arrowheads in A and C point to focal adhesions and fibrillary structures, respectively. Bar, 10 μm.