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Photomicrographs of rat kidney cortex stained for AP by the cerium-based Gomori technique. Brown reaction product is present in glomeruli from LPS-treated animals after 6 h a, arrows, and c, whereas glomeruli of control rats stain negative, b and d. Sections were incubated with either β-gP, a and b, or endotoxin, c and d, as a substrate. In addition to glomerular reaction product, up-regulation of tubular AP can be seen, a and c vs. b and d; arrowheads. Final magnification × 300.
